BioLogicReactor

Introduction:

BLRlogo.PNG

The BioLogicReactor is an incredibly cheap bio-reactor for doing experiments with bacterial, phage (and possibly yeast) cultures. By substituting computation and feedback for precision components and calibration, I'm reducing the cost of doing certain long-term (days to weeks) experiments in artificial evolution.

Cf. A System for the Continuous Directed Evolution of Biomolecules

I'm using a cheap USB camera to detect liquid levels in flasks, monitor turbidity in cell culturess, and (new) detecting bio-luminscence markers. To see the OpenCV-based level-detection at work, see: http://www.youtube.com/watch?v=46yMeK5wDpQ


Hardware:

  • 1 Raspberry Pi
  • 1 Logictech USB Webcam
  • 1 Ardweeny
  • 20 (or so) 3D printed parts (Clicking the link will download an MS Word Doc with part descriptions, images, and explanations.)
    • 4 nylon septa
    • 12 silicon tubing compression valves
    • 1 valve actuator
    • 2 motor mounts
  • 4 Peristaltic pumps
  • 3 Solenoid valves
  • 1 Heat Gun
  • 1 LM35-DZ Temperature sensor
  • 1 10-Amp Solid-state relay
  • 2 Styrofoam ice chests
  • 1 Stainless-steel tray
  • 1 Gearhead motor (Jameco 161382)
  • 1 Gearhead motor (Jameco 155855)

Software:

Raspian (Wheezy) Linux
Arduino IDE
Python
OpenCV
SWI-Prolog


3d-printing biology engineering evolution microbiology

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